所属部门：金沙威尼斯欢乐娱人城

　　职务/职称：教授

　　荣誉：陕西省中青年科技创新领军人才入选者，陕西省特支计划人选，博士生导师

　　电子邮件：wangzhf@nwu.edu.cn

　　个人简介

　　王仲孚，博士，教授，博士生导师。1995年本科毕业于西北农林科技大学，2001年博士毕业于华南理工大学生物科学与工程研究中心，2002-2004年在美国新罕布什尔大学(UNH)结构生物学研究中心(CSB)作博士后研究，2004年回国到金沙威尼斯欢乐娱人城生命科学学院工作，2018年至今在金沙威尼斯欢乐娱人城工作，现为金沙威尼斯欢乐娱人城糖生物学与糖工程研究中心主任。主要从事多糖/寡糖的结构与功能研究，主要工作包括基于生物质谱技术的高灵敏度分析和鉴定寡糖(链)的新方法和新技术的建立及应用;多糖精细结构解析及活性研究;多糖的可控性降解及其活性寡糖片段的分离制备和序列结构分析等。在国内外学术期刊发表SCI论文86篇，申请国家发明专利12项，参编学术著作1部。主持国家"863"计划项目、国家自然科学基金、国家重点研发计划子课题等国家级科研项目8项，省部级科研项目5项，横向项目1项。2015年获首届张树政糖科学奖优秀青年奖，2016年入选陕西省中青年科技创新领军人才，2017年入选陕西省高层次人才特殊支持计划科技创新领军人才，2017年获陕西高等学校科学技术奖一等奖1项(1/11)。

　　研究成果

　　1. Yangni Deng, Qian Liu, Tiantian Dang, Guiping Gong, Xiaoyi Chen, Rui Tang, Jing Sun, Shuang Song Linjuan Huang*, Zhongfu Wang *. Preparation, Structural Characterization and Bioactivity of 4-O-Methylglucuronoxylan from Artemisia sphaerocephala Krasch. Carbohydrate Polymers (2019), 222: 115009.

　　2. Tang Rui, Chen Xiaoyi, Dang Tiantian, Deng Yangni, Zou Zihua, Liu Qian, Gong Guiping, Song Shuang, Ma Fangli, Huang Linjuan*, Wang Zhongfu*. Lycium barbarum Polysaccharides Extent the Mean Lifespan of Drosophila melanogaster. Food & Function (2019), 10: 4231–4241.

　　3. Zhang Ying, Wang Bo, Jin Wanjun, Wen Yanan, Nan Lijing, Yang Mingming, Liu Rendan, Zhu Yuyang, Wang Chengjian, Huang Linjuan, Song Xuezheng*, Wang Zhongfu*. Sensitive and robust MALDI-TOF-MS glycomics analysis enabled by Girard's reagent T on-target derivatization (GTOD) of reducing glycans. Analytica Chimica Acta (2019), 1048: 105-114.

　　4. Chen Xiaoyi, Tang Rui, Liu Tingting, Dai Wei, Liu Qian, Gong Guiping, Song Shuang, Hu Minghua, Huang Linjuan*, Wang Zhongfu*. Physicochemical properties, antioxidant activity and immunological effects in vitro of polysaccharides from Schisandra sphenanthera and Schisandra chinensis. International Journal of Biological Macromolecules (2019), 131: 744–751.

　　5. Lu Yu, Li Cheng, Wei Ming, Jia Yue, Song Jingjing, Zhang Ying, Wang Chengjian, Huang Linjuan*, Wang Zhongfu*. Release, Separation, and Recovery of Monomeric Reducing N‑Glycans with Pronase E Combined with 9‑Chloromethyl Chloroformate and Glycosylasparaginase. Biochemistry (2019), 58: 1120−1130.

    6. Nan Lijing, Li Jiao, Jin Wanjun, Wei Ming, Tang Mengjun, Wang Chengjian, Gong Guiping, Huang Linjuan, Zhang Ying* and Wang Zhongfu*. Comprehensive quali-quantitative profiling of neutral and sialylated O-glycome by mass spectrometry based on oligosaccharide metabolic engineering and isotopic labeling.RSC Advances (2019), 9: 15694–15702.

　　7. Li Cheng, Wang Chengjian, Jin Wanjun, Han Jianli, Yang Meifang, Gao Xi, Huang Linjuan, Wang Zhongfu*. Mass Spectrometric Analysis of N-Glycans of Glycoprotein Separated by SDS-PAGE Gel from Ginkgo Seed. Chemical Journal of Chinese Universities (2019), 40(1): 69-75.

　　8. Lu Yu, Wang Chengjian, Liu Rendan, Jin Wanjun, Wen Yanan, Huang Linjuan*, Wang Zhongfu*.3-Amino-1-phenyl-2-pyrazoline-5-ketone as a heterobifunctional chromogenic reagent to derivatize reducing glycans for subsequent online LC/MS analysis. Analytical Biochemistry (2018), 549: 1-11.

　　9. Wang Chengjian, Yang Meifang, Gao Xi, Li Cheng, Zou Zihua, Han Jianli, Linjuan Huang* Wang Zhongfu*. The ammonia-catalyzed release of glycoprotein N-glycans, Glycoconjugate Journal (2018), 35: 411–420.

　　10. Wang Chengjian, Qiang Shan, Jin Wanjun, Song Xuezheng, Zhang Ying, Huang Linjuan, Wang Zhongfu*. Reductive chemical release of N-glycans as 1-amino-alditols and subsequent 9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis. Journal of Proteomics (2018), 187: 47–58.

　　11. Wang Chengjian, Lu Yu, Han Jianli, Jin Wanjun, Li Lingmei, Zhang Ying, Song Xuezheng, Huang Linjuan, Wang Zhongfu*. Simultaneous Release and Labeling of O- and N‑Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Journal of Proteome Research (2018), 17: 2345−2357.

　　12. Gong Guiping, Dang Tiantian, Deng Yangni, Han Jianli, Zou Zihua, Jing Sun, Zhang Ying, Liu Qian, Huang Linjuan, Wang Zhongfu*. Physicochemical properties and biological activities of polysaccharides from Lycium barbarum prepared by fractional precipitation. International Journal of Biological Macromolecules (2018), 109: 611-618.

　　13. Gong Guiping, Zhao Jixiang, Wang Chengjian, Wei Ming, Dang Tiantian, Deng Yangni, Sun Jing, Song Shuang, Huang Linjuan*, Wang Zhongfu*. Structural characterization and antioxidant activities of the degradation products from Porphyra haitanensis polysaccharidesProcess Biochemistry (2018), 74: 185–193.

　　14. Li Lingmei, Wang Changjian, Han Jianli, Liu Rendan, Wei Ming, Jin Wangjun, Huang Linjuan, Wang Zhongfu*. Mass spectrometric analysis of N-glycosylation sites and N-glycans of β-conglycinin.Chemical Journal of Chinese Universities (2018), 39(3): 427-434.

　　15. Yang Mingming, Nan Lijing, Jin Wanjun, Wang Chengjian, Huang Linjuan, Zhang Ying*, Wang Zhongfu*. Quali-quantitative Profiling of Mucin-type O-Glycans in Normal and Tumor Cell Lines Based on Oligosaccharide Metabolic Engineering Combined with Mass Spectrometry.Chemical Journal of Chinese Universities (2018), 39(9):1904-1912.

　　16. Sun Guosheng, Wu Yue, Liu Anqi, Qiu Saifeng, Zhang Wan, Wang Zhongfu, Zhang Jianbo. Substoichiometric FeCl3Activation of Propargyl Glycosides for the Synthesis of Disaccharides and Glycoconjugates. Synlett (2018), 29(5): 668-672.

　　17. Sun Yujiao, Gong Guiping, Guo Yiming, Wang Zhongfu, Song Shuang, Zhu Beiwei, Zhao Lili, Jiang Jinju. Purification, structural features and immunostimulatory activity of novel polysaccharides from Caulerpa lentillifer. International Journal of Biological Macromolecules (2018), 108: 314-323.

　　18. Shang Jiao, Fang Minfeng, Zhang Li, Wang Hui, Gong Guiping, Wang Zhongfu, Zhao Ajing, Yi Huihui. Purification and activity characterization of polysaccharides in the medicinal lichen Umbilicaria tornata from Taibai Mountain, China. Glycoconjugate Journal (2018), 35(1):107-117.

　　19. Song Shuang, Zhang Bao, Wu Sufeng, Huang Lu, Ai Chunqing, Pan Jinfeng, Su Yi-Cheng, Wang Zhongfu, Wen Chengrong. Structural characterization and osteogenic bioactivity of a sulfated polysaccharide from pacific abalone (Haliotis discus hannai Ino). Carbohydrate Polymers(2018), 182: 207-214.

　　20. Wei Jinhua, Wang Zhuo A, Wang Bing, Jahan Marefa, Wang Zhongfu, Du Yuguang, Wynn Peter C. Characterization of porcine milk oligosaccharides over lactation between primiparous and multiparous female pigs. Scientific reports (2018), 8(1): 4688.

　　21. Wang Jianhui, Cheng Xiaorui, Zeng Ju, Zhou Wenxia, Zhang Yongxiang, Yuan Jiangbei, Wang Zhongfu. LW-AFC Effects on N-glycan Profile in Senescence-Accelerated Mouse Prone 8 Strain, a Mouse Model of Alzheimer's Disease. Aging and disease (2017), 8(1):101-114.

　　22. Tan Shuguang, Zhang Hao, Chai Yan, Song Hao, Tong Zhou, Wang Qihui, Qi Jianxun, Wong Gary, Zhu Xiaodong, Liu William J, Gao Shan, Wang Zhongfu, Shi Yi, Yang Fuquan, Gao George F, Yan Jinghua. An unexpected N-terminal loop in PD-1 dominates binding by nivolumab. Nature Communications (2017), 8: 14369.

　　23. Wang Chengjian, Zhang Ping, Jin Wanjun, Li Lingmei, Qiang Shan, Zhang Ying, Huang Linjuan*, Wang Zhongfu*. Quantitative O-glycomics based on improvement of the one-pot method for nonreductive O-glycan release and simultaneous stable isotope labeling with 1-(d0/d5)phenyl-3-methyl-5-pyrazolone followed by mass spectrometric analysis. Journal of Proteomics (2017), 150: 18-30.

　　24. Sun Guosheng, Qiu Saifeng, Ding Zekun, Chen Heshan, Zhou Jiafen, Wang Zhongfu, Zhang Jianbo. Magnetic Core-Shell Fe3O4@C-SO3H as an Efficient and Renewable 'Green Catalyst' for the Synthesis of O-2, 3-Unsaturated Glycopyranosides. Synlett (2017), 28(3), 347-352.

　　25. Zhang Wan, Luo Xiaosheng, Wang Zhongfu, Zhang Jianbo. One-pot synthesis of β-2,6-dideoxyglycosides via glycosyl iodide intermediates. Journal of Carbohydrate Chemistry (2016), 35(6): 315-325.

　　26. Peng Yifang, Wang Chengjian, Wang Jingjing, Li Lingmei, Jin Wanjun, Qiang Shan, Shi Hongdan, Zhang Ying, Huang Linjuan, Wang Zhongfu*. Analysis of antigenic determinant glycans of peanut allergy glycoprotein Ara h1 by mass spectrometry. Gaodeng Xuexiao Huaxue Xuebao(2016), 37(9): 1622-1628.

　　27. Qiu Saifeng, Zhang Wan, Sun Guosheng, Wang Zhongfu, Zhang Jianbo. A Facile and Direct Glycosidation Method for the Synthesis of 2-Deoxy α-Rhamnosides Catalyzed by Ferric Chloride. ChemistrySelect (2016), 1(15): 4840-4844.

　　28. Li Lingmei, Wang Chengjian, Qiang Shan, Zhao Jixiang, Song Shuang, Jin Wanjun, Wang Bo, Zhang Ying, Huang Linjuan*, Wang Zhongfu*. Mass Spectrometric Analysis of N-Glycoforms of Soybean Allergenic Glycoproteins Separated by SDS-PAGE. Journal of Agricultural and Food Chemistry (2016), 64(39): 7367-7376.

　　29. Chen Zhina, Shi Junling, Yang Xijuan, Liu Yang, Nan Bo, Wang Zhongfu. Isolation of exopolysaccharide-producing bacteria and yeasts from Tibetan kefir and characterisation of the exopolysaccharides.International Journal of Dairy Technology (2016), 69(3): 410-417.

　　30. Chen Heshan, Xian Ting, Zhang Wan, Si Wenshuai, Luo Xiaosheng, Zhang Bo, Zhang Meiyu, Wang Zhongfu, Zhang Jianbo. An efficient method for the synthesis of pyranoid glycals. Carbohydrate Research(2016), 431: 42-46.

　　31. Bai Liang, Li Qianwei, Li Lingmei, Lin Yan, Zhao Sihai, Wang Weirong, Wang Rong, Li Yongqin, Yuan Jiangbei, Wang Chengjian, Wang Zhongfu*, Fan Jianglin, Liu Enqi*. Plasma high-mannose and complex/hybrid N-glycans are associated with hypercholesterolemia in humans and rabbits. PLoS One (2016), 11(3): e0146982/1 -e0146982/16.

　　32. Liu Yang, Yin Lu, Gong Guiping, Peng Yifang, Huang Linjuan, Wang Zhongfu*. Structural characterization, antioxidant activity and immunomodulatory activity of the polysaccharide LRLP3 from leaves of Lycium ruthenicum murra. Gaodeng Xuexiao Huaxue Xuebao (2016), 37(2): 261-268.

　　33. Liu Yang, Gong Guiping, Sun Yujiao, Gu Xiao, Huang Linjuan, Wang Zhongfu*. Isolation, structural characterization, and immunological activity of a polysaccharide LRLP4-A from the leaves of Lycium ruthenicum. Journal of Carbohydrate Chemistry (2016), 35(1), 40-56.

　　34. Gong Guiping, Fan Jiangbo, Sun Yujiao, Wu Yanmin, Liu Yang, Sun Wei, Zhang Ying*, Wang Zhongfu*. Isolation, structural characterization, and antioxidativity of polysaccharide LBLP5-A from Lycium barbarum leaves. Process Biochemistry (2016), 51(2): 314-324.

　　35. Zhang Ying，Liu Ying，Liu Yang，Qiang Shan，Yang Bingying，Huang Linjuan，Wang Zhongfu*. Pretreatment Method for Detecting of N-linked Glycans of Glycoprotein in Micro-scale Complex Biological Sample based on Electrospray Ionization Mass Spectrometry. Chinese Journal of Analytical Chemistry (2016), 44(2): 265-272.

　　36. Lu Hua, Lu Yang, Ren Jinwei, Wang Zhongfu, Wang Qian, Luo Yuanming, Han Jing, Xiang Hua, Du Yuguo, Jin Cheng. Identification of the S-layer glycoproteins and their covalently linked glycans in the halophilic archaeon Haloarcula hispanica. Glycobiology (2015), 25(11), 1150-1162.

　　37. Pan Liying, Gu Xiao, Wang Chengjian, Qiang Shan, Huang Linjuan, Zhang Ying*, Wang Zhongfu*. Comparative analysis of O-glycans from human hepatocellular carcinoma HepG2 and normal liver cells L02.Gaodeng Xuexiao Huaxue Xuebao (2015), 7: 1275-1281.

　　38. Li Wenzhe, Yu Rui, Ma Biao, Yang Yan, Jiao Xinyan, Liu Yang, Cao Hongyu, Dong Weijie, Liu Linhua, Ma Keli, Fukuda Tomohiko, Liu Qingping, Ma Tonghui, Wang Zhongfu, Gu Jiangguo, Zhang Jianing, Taniguchi Naoyuki. Core Fucosylation of IgG B Cell Receptor Is Required for Antigen Recognition and Antibody Production. Journal of Immunology (2015), 194(6): 2596-2606.

　　39. Sun Yujiao, Yang Bingying, Wu Yanmin, Liu Yang, Gu Xiao, Zhang Hong, Wang Chengjian, Cao Hongzhi, Huang Linjuan*, Wang Zhongfu*. Structural characterization and antioxidant activities of κ-carrageenan oligosaccharides degraded by different methods. Food Chemistry (2015), 178: 311-318.

　　40. Sun Yujiao, Sun Wei, Guo Jiatong, Hu Xiuting, Gong Guiping, Huang Linjuan, Cao Hongzhi, Wang Zhongfu*. Sulphation pattern analysis of chemically sulphated polysaccharide LbGp1 from Lycium barbarum by GC–MS. Food Chemistry (2015), 170: 22–29.

　　41. Chen Zhina, Shi Junling, Yang Xijuan, Nan Bo, Liu Yang, Wang Zhongfu. Chemical and physical characteristics and antioxidant activities of the exopolysaccharide produced by Tibetan kefir grains during milk fermentation. International Dairy Journal (2015), 43: 15-21.

　　42. Pan Liying, Wang Chengjian, Yuan Jiangbei, Zhang Ying, Huang Linjuan, Wang Zhongfu*. Qualification and quantification for comparative analysis of N-glycans from human hepatocellular carcinoma HepG2 and normal liver cells L02 by electrospray ionization mass spectrometry. Gaodeng Xuexiao Huaxue Xuebao (2014), 35(2): 237-243.

　　43. Yuan Jiangbei, Wang Chengjian, Sun Yujiao, HuangLinjuan*, Wang Zhongfu*. Nonreductive chemical release of intact N-glycans for subsequent labeling and analysis by mass spectrometry. Analytical Biochemistry(2014), 462: 1-9.

　　44. Zhang Ying, Wang Chengjian, Liu Yang, Yao Weinan, Sun Yujiao, Zhang Ping, Huang Linjuan, Wang Zhongfu*. Fluorescein-5-thiosemicarbazide (FTSC) labeling for fluorescent imaging of pectin-derived oligogalacturonic acid transported in living cells by confocal microscopy. European Food Research and Technology (2014), 239(5): 867-875.

　　45. Jiang Kuan, Wang Chengjian, Sun Yujiao, Liu Yang, Zhang Ying, Huang Linjuan*, Wang Zhongfu*. Comparison of Chicken and Pheasant Ovotransferrin N-Glycoforms via Electrospray Ionization Mass Spectrometry and Liquid Chromatography Coupled with Mass Spectrometry. Journal of Agricultural and Food Chemistry (2014), 62(29): 7245-7254.

　　46. Xue Xiangdong, Sun Yujiao, Liu Yang, Zhang Ping, Wang Zhongfu, Huang Linjuan. A novel method for relative quantitation of N-glycans via acetone stable isotopic labeling and ESI-MS analysis. Huaxue Xuebao (2014), 72(2): 220-226.

　　47. Wang Zhongfu, Liu Yang, Sun Yujiao, Mou Qing, Wang Bo, Zhang Ying, Huang Linjuan. Structural characterization of LbGp1 from the fruits of Lycium barbarum L. Food Chemistry (2014), 159: 137-142.

　　48. Sun Yujiao, Liu Yang, Jiang Kuan, Wang Chengjian, Wang Zhongfu, Huang Linjuan. Electrospray Ionization Mass Spectrometric Analysis of κ-Carrageenan Oligosaccharides Obtained by Degradation with κ-Carrageenase from Pedobacter hainanensis. Journal of Agricultural and Food Chemistry (2014), 62(11), 2398-2405.

　　49. Wang Chengjian, Wu Zhiyu, Yuan Jiangbei, Wang Bo, Zhang Ping, Zhang Ying, Wang Zhongfu*, Huang Linjuan*. Simplified Quantitative Glycomics Using the Stable Isotope Label Girard's Reagent P by Electrospray Ionization Mass Spectrometry. Journal of Proteome Research (2014), 13(2): 372-384.

　　50. Zhang Hong, Wang Hai-Feng, Liu Yang, Huang Linjuan, Wang Zhongfu*, Li Ye. The haematopoietic effect of Panax japonicus on blood deficiency model mice. Journal of Ethnopharmacology (2014), 154: 818–824.

　　51. Zhang Jianbo, Zhang Bo, Zhou Jiafen, Chen Heshan, Li Juan, Yang Guofang, Wang Zhongfu, Tang Jie. A Facile H2SO4-SiO2-Catalyzed Ferrier Rearrangement of 3,4,6-Tri-O-benzyl-d-glucal. Journal of Carbohydrate Chemistry (2013), 32(5-6): 380-391.

　　52. Wang Chengjian, Yuan Jiangbei, Li Xiaohua, Wang Zhongfu*, Huang Linjuan*. Sulfonyl hydrazine-functionalized polymer as a specific capturer of reducing glycans from complex samples for high-throughput analysis by electrospray ionization mass spectrometry. Analyst (2013), 138(18): 5344-5356.

　　53. Zhang Ying, Yuan Jiangbei, Song Jingjing, Wang Zhongfu*, Huang Linjuan*. An efficient method for selectively imaging and quantifying in situ the expression of sialylated glycoproteins on living cells.Glycobiology(2013), 23(6): 643-653.

　　54. Li Qian, Zhou Siyuan, Jing Juan, Yang Tiehong, Duan Shuangyan, Wang Zhongfu, Mei Qibing, Liu Li. Oligosaccharide from apple induces apoptosis and cell cycle arrest in HT29 human colon cancer cells. International Journal of Biological Macromolecules (2013), 57: 245-254.

　　55. Wang Chengjian, Yuan Jiangbei, Wang Zhongfu*, Huang Linjuan*. Separation of one-pot procedure released O-glycans as 1-phenyl-3-methyl-5-pyrazolone derivatives by hydrophilic interaction and reversed-phase liquid chromatography followed by identification using electrospray mass spectrometry and tandem mass spectrometry. Journal of Chromatography A (2013), 1274: 107-117.

　　56. Lv Xiaopeng, Wang Chengjian, Cheng Yang, Huang Linjuan*, Wang Zhongfu*. Isolation and structural characterization of a polysaccharide LRP4-A from Lycium ruthenicum Murr. Carbohydrate Research(2013), 365: 20-25.

　　57. Peng Qiang, Song Jingjing, Lv Xiaopeng, Wang Zhongfu, Huang Linjuan, Du Yuguang. Structural Characterization of an Arabinogalactan-Protein from the Fruits of Lycium ruthenicum. Journal of Agricultural and Food Chemistry (2012), 60(37): 9424-9429.

　　58. Zhang Ying, Sun Yujiao, Wang Zhongfu*, Huang Linjuan*. Fluorescein-5-thiosemicarbazide as a probe for directly imaging of mucin-type O-linked glycoprotein within living cells. Glycoconjugate Journal (2012), 29(5-6): 445-452.

　　59. Zhang Ying, Liu Yang Wang Zhijun, Wang Zhongfu, Huang Linjuan. The synthesis of a 2-deoxy-2-acetonyl sugar from its corresponding natural saccharide. Journal of Chemical Research (2012), 36(4): 244-246.

　　60. Wang Chengjian, Fan Wancui, Zhang Ping, Wang Zhongfu*, Huang Linjuan*. One-pot nonreductive O-glycan release and labeling with 1-phenyl-3-methyl-5-pyrazolone followed by ESI-MS analysis. Proteomics (2011), 11(21), 4229-4242.

　　61. Zhang Jianbo, Zhang Bo, Zhou Jiafen, Li Juan, Shi Chunjuan, Huang Ting, Wang Zhongfu, Tang Jie. H2SO4-SiO2: Highly Efficient and Reusable Catalyst for per-O-Acetylation of Carbohydrates Under Solvent-Free Conditions. Journal of Carbohydrate Chemistry (2011), 30(3): 165-177.

　　62. Zhang Ying, Zhang Ping, Wang Zhongfu, Huang Linjuan. An Innovative Derivatization Method for Simultaneous Determination of Uronic Acids and Neutral and Amino Sugars in Coexisting Samples by HPLC-ESI-MS/MS2. Journal of Liquid Chromatography & Related Technologies (2011), 34(16):1754-1771.

　　63. Zhang Jianbo, Fu Jie, Si Wenshuai, Wang Xiaohu, Wang Zhongfu*, Tang Jie. A highly efficient deprotection of the 2,2,2-trichloroethyl group at the anomeric oxygen of carbohydrates.Carbohydrate Research (2011), 346(14): 2290-2293.

　　获奖情况

　　1.《寡糖/多糖分析鉴定方法和技术体系的建立及应用》，陕西高等学校科学技术奖，一等奖，王仲孚(1/11)。(2017)

　　2. 入选陕西省高层次人才特殊支持计划科技创新领军人才。(2017)

　　3. 入选陕西省创新人才推进计划“中青年科技创新领军人才”(2016)

　　4. 首届“张树政糖科学奖•优秀青年奖”获得者(2015)